ABSTRACT
Paciente de 43 años diagnosticado con leucemia mieloide crónica en 1998, que fue tratado de forma inicial con interferón alfa. En la terapia posterior adquirió múltiples cambios citogenéticos clonales en células del cromosoma Filadelfia negativas, por lo que se describe el efecto de los inhibidores de la tirosina quinasa de segunda generación sobre esos clones celulares.
Subject(s)
Humans , Male , Adult , Clone Cells/physiology , Clone Cells/metabolism , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/complications , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy , Protein-Tyrosine Kinases/administration & dosage , Protein-Tyrosine Kinases/pharmacology , Protein-Tyrosine Kinases/therapeutic useABSTRACT
An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo developmental capacities of NT using fibroblast cells isolated from a 14-month-old cloned Simmental heifer (FCE) vs the same line transfected with a plasmid containing neomycin-resistant genes (TFCE). There were no significant differences (P > 0.5) in either fusion (116/149 = 78% vs 216/301 = 72%), cleavage (78/116 = 67% vs 141/216 = 65%) and blastocyst (35/116 = 30% vs 52/216 = 24%) rates or in pregnancy rate at 30 to 35 days after embryo transfer (2/17 vs 3/17) between NT using FCE and TFCE, respectively. Transfection and long-term in vitro culture of transfected cells did not affect developmental capacity of NT embryos up to 40 days of gestation
Subject(s)
Animals , Female , Pregnancy , Animals, Genetically Modified , Cattle/genetics , Embryo Transfer , Fibroblasts/transplantation , Cell Nucleus/transplantation , Blastocyst/physiology , Cloning, Organism , Clone Cells/physiology , Polymerase Chain Reaction , Transfection/methodsABSTRACT
Se investigó la actividad hemolítica de 11 cepas y 6 clones vivos de trichomonas vaginalis. Las cepas y los clones fueron subsecuentemente estudiados frente a los eritrocitos de 7 especies animales adultas. Cada una da 11 cepas y 6 clones lisó los eritrocitos de todos los grupos sanguineos humanos, lo mismo que los de conejo, rata, pollo, caballo, bovino y ovino. No se detectó la liberación de hemolisina por el parásito (cepas y clones). Los resultados sugieren que la acividad hemolítica no es debida a la liberación de hemolisina por la T. vaginalis o por un producto de su metabolismo